Purification and differentiation of three avian adenoviruses by restriction endonuclease analysis.
No Thumbnail Available
Date
2000-02-24
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A rapid method of ultracentrifugation pelleting of avian adenovirus (AAV) from small volume of chloroform treated infected cell culture fluid or allantoic fluid was adapted for isolation of adenoviral DNA. The viral DNA extracted from semipurified viruses was found to be intact on agarose gel and pure enough (A260/280 = 1.85-1.92) for restriction enzyme analysis. Restriction endonuclease analysis of Indian strain of AAV serotype 1, AAV serotype 4 (group I AAVs) and egg drop syndrome-76 (EDS-76) virus genomes (group III AAV) with Hind III enzyme differentiated these viruses. The AAV serotype 1 and serotype 4 strain exhibited identical Hind III profile to European viral strains belonging to same serotypes however, the EDS-76 virus gave similar but not identical profile. The calculated genomic lengths for AAV serotype 1 and EDS-76 virus were approximately found to be 33.9 and 44.4 Kb, respectively.
Description
Keywords
Citation
Jadhao SJ, Kataria JM, Deepak JN, Verma KC, Kataria RS, Tiwari AK. Purification and differentiation of three avian adenoviruses by restriction endonuclease analysis. Indian Journal of Experimental Biology. 2000 Feb; 38(2): 186-8