Detection of autoreactive antibodies to serum IgG and IgA in amoebic liver abscess cases.
No Thumbnail Available
Date
1992-06-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Assessment of autoreactive antibodies in response to healthy human serum IgA and IgG was performed by indirect haemagglutination assay on serum samples from 81 amoebic liver abscess cases for IgA and 70 for IgG. Appropriate controls were taken simultaneously. IgA, IgG were isolated and purified from a healthy human serum through Sephadex G-200 and protein A CL 4B sepharose chromatography. These immunoglobulins were used for the detection of its own antibodies in amoebic liver abscess cases. This revealed that 43.20% and 48.50% of the cases were positive for IgA and IgG respectively, where as only 19.35% and 28.30% of the controls were in positive category (IgA and IgG respectively). The mean titres with standard deviation of the autoreactive antibodies to serum IgA both in ALA cases and controls shows a highly significant difference between tests and controls (P less than 0.001). Similarly the mean titres with standard deviation both in ALA and controls for the serum IgG differed significantly (P less than 0.001). This suggests the presence of autoreactive antibodies against serum IgA and IgG in amoebic liver abscess cases.
Description
Keywords
Citation
Nayeem MA, Ishaq M, Habibullah CM, Begum S. Detection of autoreactive antibodies to serum IgG and IgA in amoebic liver abscess cases. Indian Journal of Experimental Biology. 1992 Jun; 30(6): 549-51