In vitro propagation of emetic nut Randia dumetorum (Lamb.).
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2003-12-24
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An efficient protocol for in vitro shoot multiplication of Randia dumetorum (Emetic nut) has been developed. The seeds of R. dumetorum were germinated in vitro in MS medium in 5 weeks. Subsequent propagation using shoot tip as an explant was carried out in MS medium along with different concentrations and combinations of BAP (0.5-2.0) and NAA (0.0-2.0). Maximum shoot multiplication was obtained (12.7 shoots per shoot tip) in MS medium containing 1 mg/L BAP and 1 mg/L NAA. Micropropagated shoots were rooted in 1/2 MS medium supplemented with 1 mg/l IBA. This is the first report of in vitro plant propagation of R. dumetorum. In vitro grown plantlets showed a survival rate of 70% after 2 months of transplantation to natural environment.
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Begum F, Islam KM, Paul RN, Mehedi M, Rani S. In vitro propagation of emetic nut Randia dumetorum (Lamb.). Indian Journal of Experimental Biology. 2003 Dec; 41(12): 1479-81