New blood free biphasic medium with haemoglobin for cultivation of Leishmania donovani promastigotes.
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1996-12-01
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A simple biphasic growth medium for cultivation of L. donovani promastigotes is described. A successful attempt has been made to replace the blood/serum from regular medium by bovine hemoglobin powder. Medium is easy to prepare within 4 hr. It is simple, reliable inexpensive and autoclavable. Ingredients used in the media preparation are readily available at low cost and components of the medium prepared in large quantities, could be stored at 4 degrees C for month without significantly altering their growth supporting potential. After an inoculation of 1 x 10(5) promastigotes/ml, it was possible to cultivate 1-3 x 10(2) promastigotes/ml from the above described media.
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Agarwal M, Jain A. New blood free biphasic medium with haemoglobin for cultivation of Leishmania donovani promastigotes. Indian Journal of Experimental Biology. 1996 Dec; 34(12): 1233-6