Induction of acrosome reaction in fresh and frozen-thawed bovine spermatozoa by platelet activating factor.
No Thumbnail Available
Date
1995-02-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
In vitro capacitation has been induced in fresh and frozen spermatozoa of Karan Swiss (KS) and Karan Fries (KF) crossbred cattle by using a phospholipid-platelet activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine). The capacitation was monitored by examining acrosome reaction (AR) and motility at various levels of platelet activating factor (PAF) and at the end of each incubation time. On an average, with the increase in incubation time, there was a gradual decrease in motility and increase in acrosome reaction in both fresh as well as frozen spermatozoa. As PAF level increased, the motility of fresh sperms decreased and their acrosome reaction increased dramatically. However, in frozen-thawed semen, the motility remained almost the same and increase in AR of frozen spermatozoa was not pronounced. PAF level of about 100 microM was observed to be most optimum as at this level AR improved significantly without much loss of motility.
Description
Keywords
Citation
Aravindakshan TV, Sharma A. Induction of acrosome reaction in fresh and frozen-thawed bovine spermatozoa by platelet activating factor. Indian Journal of Experimental Biology. 1995 Feb; 33(2): 87-90