Expression and stable germline transmission of neomycin-resistance gene in transgenic mice.
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1998-12-27
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Transgenic mice were produced to study the expression of amino-3' glycosyl phosphotransferase gene (neomycin resistance gene) in the embryonic fibroblast cells. A 1.9 Kb linear fragment of neomycin resistance gene under the control of pPGK promoter was microinjected into the pronucleus of mouse embryos. Out of 64 potential founders born, 5 were identified to be transgenic by the polymerase chain reaction (PCR) and southern hybridization. Multiple mice from first and second generation from two transgenic founders (N-10 and N-32) were analysed to determine the germline transmission. It was found to be 24.6 and 71.4% in first and second generation respectively. Results were also further confirmed by RT-PCR, sequencing and in vitro bioassays.
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Kaur G, Gulati N, Mandal A, Babu DS, Anand R, Garg L, Totey SM. Expression and stable germline transmission of neomycin-resistance gene in transgenic mice. Indian Journal of Experimental Biology. 1998 Dec; 36(12): 1209-15