Analysis and potential of excretory-secretory antigens of Setaria cervi for immunodiagnosis of human filariasis.
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1996-06-01
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The excretory-secretory (E-LS) products released by the adult Setaria cervi, a bovine filarial parasite, were used to raise polyvalent hyperimmune serum in rabbits. Analysis of E-S products, using anti-E-S serum showed the presence of 10-14 immunogenic proteins, the rabbit anti-E-S serum showed reciprocal antibody titres in the range of 100,000-250,000 by enzyme linked immunosorbent assay. The anti-E-S antibodies could detect circulating antigen in filarial patients sera by Counter immunoelectrophoresis.
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Mustafa H, Srivastava N, Kaushal DC, Kaushal NA. Analysis and potential of excretory-secretory antigens of Setaria cervi for immunodiagnosis of human filariasis. Indian Journal of Experimental Biology. 1996 Jun; 34(6): 508-12