Cloning of extracellular lipase gene from Xanthomonas campestris pathovar sesami on to Escherichia coli.
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1996-01-01
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Abstract
A lipase gene from X. campestris pv. sesami (strain XcS 1) causal agent of leaf spot disease of Sesamum indicum, was cloned onto E. colt. XcS showed the presence of lip+ transformants on Dye's medium with 1% glycerol as sole carbon source. The recombinant plasmids were isolated and when digested with Eco R1, yielded 2 fragments with molecular weights 4.0 and 4.8 kb. Thus a 8.8 kb insert DNA fragment was obtained which showed lipase activity.
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Sheela P, Amuthan G, Mahadevan A. Cloning of extracellular lipase gene from Xanthomonas campestris pathovar sesami on to Escherichia coli. Indian Journal of Experimental Biology. 1996 Jan; 34(1): 27-31