Studies on cytochrome oxidase in carbon tetrachloride treated rats.
No Thumbnail Available
Date
1999-11-28
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Cytochrome c oxidase was purified from control and CCl4 treated rats and its kinetic properties were studied. The activity of the enzyme was inhibited by 51% in CCl4 (4 g per kg body weight for 24 hr) treated rats. Studies on the kinetic properties showed that the K(m) of the enzyme increased by 60% while Vmax decreased by 44% in CCl4 treated rats compared to controls. The content of cytochrome aa3 was decreased by 34% while cytochrome b and c were not affected by CCl4 treatment. Phosphatidylcholine, phosphatidylethanolamine and cardiolipin were decreased significantly by 40%, 49% and 60% respectively in CCl4 treated rats. A decrease in the cytochrome aa3 content and a change in the lipid environment of the membrane are probably responsible for a decreased rate of electron transfer from cytochrome c to oxygen.
Description
Keywords
Citation
Padma P, Setty OH. Studies on cytochrome oxidase in carbon tetrachloride treated rats. Indian Journal of Experimental Biology. 1999 Nov; 37(11): 1139-41