Isolation and subcloning of chitinase clone from chickpea genomic library.
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1996-06-01
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Chickpea genomic library constructed earlier in phage lambda (EMBL-3) was screened for the presence of chitinase clone using tobacco chitinase cDNA as a probe. Positive clones obtained by primary screening of plaques (2 x 10(6)) were ascertained by secondary and tertiary screening. Presence of chitinase insert in the positive clones obtained, was further confirmed by restricting phage DNA with Sal I and then doing southern with tobacco chitinase. The insert band was eluted out and subcloned in puc 19 plasmid.
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Grover A, Tyagi A, Koundal KR, Sharma RP. Isolation and subcloning of chitinase clone from chickpea genomic library. Indian Journal of Experimental Biology. 1996 Jun; 34(6): 594-6