Effect of hematoporphyrin derivative and light on sulfhydryl groups in brain tumour cells.
No Thumbnail Available
Date
1995-10-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Effect of hematoporphyrin derivative (HpD) and light on sulfhydryl (SH) groups in brain tumor cells was studied. Sulfhydryl groups were measured by 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) and a fluorescent probe 3,4-maleimidylphenyl-4-methyl-7-diethylaminocoumarin (CPM). Incubation of cells with HpD in dark resulted in the loss of DTNB as well as CPM reactive SH groups. After 2 hr of incubation DTNB reactive SH groups showed a negligible change while a continuous decrease was observed in CPM reactive SH groups. Cells treated with HpD showed a further degradation of SH groups upon light irradiation. A comparison of cytotoxicity and SH groups under identical conditions showed that blockage of SH groups by HpD binding is not leathal to the cells where as photoinduced cell death was observed on photodegradation of SH groups.
Description
Keywords
Citation
Joshi PG, Joshi K, Joshi NB. Effect of hematoporphyrin derivative and light on sulfhydryl groups in brain tumour cells. Indian Journal of Experimental Biology. 1995 Oct; 33(10): 721-4