Quantitative estimation of major histocompatibility complex antigens on live tumour cells.
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1990-11-01
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Abstract
Quantitation of major histocompatibility complex (MHC) antigens on cells can accurately be done by using a flowcytometer. Since flowcytometer is not freely accessable an alternate, simple method for relative quantitation of MHC antigens has been devised. In this procedure, YAC lymphoma cells were first treated with a monoclonal anticlass I MHC antibody and then with a rabbit anti mouse Ig-antibody coupled to peroxidase, followed by colour development using a substrate of peroxidase enzyme. Various assay parameters have been optimized. The validity of the procedure was examined by assessing the enhanced MHC expression on YAC cells treated with a soluble rat spleen derived factor, by the new procedure as well as by the flowcytometer. Comparable results were obtained by using both techniques.
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Sarin A, Saxena RK. Quantitative estimation of major histocompatibility complex antigens on live tumour cells. Indian Journal of Experimental Biology. 1990 Nov; 28(11): 1017-20