Rapid extraction of DNA from diverse soils by guanidine thiocyanate method.
No Thumbnail Available
Date
2001-09-08
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Molecular methods are being frequently used for the study of soil microbial communities as majority of naturally occurring microbial populations are non-culturable. In the present study, we describe a protocol of DNA extraction from diverse soils using a combination of heat, enzyme (lysozyme) and guanidine thiocyanate. The efficacy of the procedure was evaluated in terms of yield, purity and duration of extraction. The protocol was effective for neutral, acidic as well as alkaline soils (pH range 4.5-8.5). The extracted soil DNA was observed with negligible shearing on agarose gel and the time taken for restriction digestion was very less. Further, the DNA extracted was almost completely devoid of contaminants and pure enough which could be used for PCR amplification and Southern hybridization.
Description
Keywords
Citation
Agarwal A, Kumar C, Goel R. Rapid extraction of DNA from diverse soils by guanidine thiocyanate method. Indian Journal of Experimental Biology. 2001 Sep; 39(9): 906-10