Construction of a gene bank of Rhizobium leguminosarum Rld 164.
No Thumbnail Available
Date
1989-03-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The total genomic DNA of R. leguminosarum Rld164 (trp, sms, azi) was cloned in the EcoR1 site of the wide host and conjugally transferable cosmid vector pLAFR1. The average insert size in the gene clones of the bank was found to be 21.3 Kb. The strain R. leguminosarum Rld7 (leu-1) was employed as recepient to conjugally transfer and thus isolate the complementary leu+ allele carrying clones from the gene bank.
Description
Keywords
Citation
Stanfield S, Singh A, Kashyap LR, Kumar S. Construction of a gene bank of Rhizobium leguminosarum Rld 164. Indian Journal of Experimental Biology. 1989 Mar; 27(3): 288-9