Metabolic studies on mycobacteria. IV. Assay of isocitrate lyase and malate synthase activity in M. leprae.
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1987-04-01
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Abstract
Cell free extracts from M. tuberculosis H37 Rv, M. smegmatis armadillo derived M. leprae and normal armadillo liver homogenates were assayed for the presence of isocitrate lyase and malate synthase activity. It was observed that significant amount of isocitrate lyase and malate synthase activity was present in M. tuberculosis H37 Rv, M. smegmatis and armadillo derived M. leprae. No such activity was demonstrable in cell free extracts of normal armadillo liver. It is concluded that M. leprae like other mycobacteria has the capability to metabolise via glyoxylate bypass of TCA cycle. These findings may be relevant for understanding the energy metabolism of M. leprae under stress conditions and possibly the 'persister' stage.
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Bharadwaj VP, Katoch VM, Sharma VD, Kannan KB, Datta AK, Shivannavar CT. Metabolic studies on mycobacteria. IV. Assay of isocitrate lyase and malate synthase activity in M. leprae. Indian Journal of Leprosy. 1987 Apr-Jun; 59(2): 158-62