Prevalence of hepatitis C virus (HCV) coinfection in HIV infected individuals in south India and characterization of HCV genotypes.
No Thumbnail Available
Date
2009-01-28
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
PURPOSE: To determine anti-HCV antibodies and genomic subtype of HCV in 1487 confirmed human immunodeficiency virus (HIV) positive samples.
METHODS: A total of 1487 confirmed HIV-positive samples were tested for anti-HCV antibodies by using a third generation ELISA kit (Ortho 3.0) and by RT PCR for HCV. HIV and HCV coinfected samples were selected for HCV genotyping by RFLP and subtyping with NS5-type specific primers.
RESULTS: A total of 1487 HIV-infected serum samples were screened for HCV infection, of which, a 1443 (97.04%) were negative and 45 (3.02%) were coinfected. HIV-HCV coinfection was predominant in the age group 41-50 years (51.1%). HCV genotyping and subtyping was done for the 45 HCV RNA-positive specimens of which genotype 1 was observed in 31 (68.8%) and genotype 3 was observed in 14 (31.1%) subjects. Further subtyping analysis showed the genotype 1b in 23 (51.1%), 1a in eight (17.7%), 3a in 10 (22.2%) and 3b in four (8.8%) subjects.
CONCLUSION: HIV and HCV seroprevalence is higher in South India, and the most prevalent genotype in coinfection was genotype 1b.
Description
Keywords
Citation
Ponamgi SP, Rahamathulla S, Kumar YN, Chandra M, Lakshmi N, Habibullah CM, Khaja MN. Prevalence of hepatitis C virus (HCV) coinfection in HIV infected individuals in south India and characterization of HCV genotypes. Indian Journal of Medical Microbiology. 2009 Jan-Mar; 27(1): 12-6