Multiplex PCR to detect the dystrophin gene deletion in Thai patients.

Mutirangura, A; Jongpiputvanich, S; Norapucsunton, T; Theamboonlers, A; Srivuthana, S; Promchainant, C; Tumwasorn, S; Sueblinvong, T

Multiplex PCR to detect the dystrophin gene deletion in Thai patients.

Date

1995-09-01

Authors

Abstract

We have demonstrated the usefulness of the multiplex PCR to directly detect the dystrophin gene mutation. Prenatal diagnosis and confirmation of clinical diagnosis of DMD/BMD via non invasive technique are now possible. Nine DMD and one BMD patients were tested. Five DMD patients demonstrated deletion. Thus, this multiplex PCR could detect deletion in approximately 50 per cent of DMD/BMD Thai patients. Eighty per cent of the deletions were in the distal part, whereas, 20 per cent were in the proximal part. We are planning to establish other molecular techniques such as linkage analysis, cDNA hybridization and immunostaining of dystrophin protein to improve a mode of diagnosis and management of DMD/BMD patients in the Thai community.

Description

Chotmaihet Thangphaet.

Citation

Mutirangura A, Jongpiputvanich S, Norapucsunton T, Theamboonlers A, Srivuthana S, Promchainant C, Tumwasorn S, Sueblinvong T. Multiplex PCR to detect the dystrophin gene deletion in Thai patients. Journal of the Medical Association of Thailand. 1995 Sep; 78(9): 460-5