Vitrification of mouse oocyte using open pulled straws compared with needles.

Pornwiroon, Surachai; Kunathikom, Somboon; Makemaharn, Orawan; Huanaraj, Rattanaphorn

Vitrification of mouse oocyte using open pulled straws compared with needles.

Date

2006-12-12

Authors

Pornwiroon, Surachai

Kunathikom, Somboon

Makemaharn, Orawan

Huanaraj, Rattanaphorn

Abstract

OBJECTIVE: To compare the survival rate of mouse oocytes and fertilization rate between using open pulled straws (OPS) and needles for vitrification. MATERIAL AND METHOD: Meiosis II oocytes from female C57B/6J mice aged 7-8 weeks were collected and allocated to two groups for vitrification by using OPS or needles. Vitrified oocytes were thawed, morphological survival and fertilization rate were examined. RESULTS: There was no obvious difference between the morphological survival rates of vitrified mouse oocytes using OPS and needles (66.7% vs 64.8%). Proportions Difference 1.9% (95% CI -7.1, 10.7). The vitrified oocytes from the needle had significantly higher percentages of fertilization rate than OPS (76.8% vs 62.5%). Proportions Difference -14.3% (95% CI -24.5, -3.6). CONCLUSION: Vitrification method of mouse oocytes using needles when compared to OPS provides a similar morphological survival rate and higher fertilization rate.

Description

Chotmaihet Thangphaet.

Citation

Pornwiroon S, Kunathikom S, Makemaharn O, Huanaraj R. Vitrification of mouse oocyte using open pulled straws compared with needles. Journal of the Medical Association of Thailand. 2006 Dec; 89(12): 2015-20