Molecular cytogenetic analysis of urothelial carcinomas using urine samples.
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Date
2008-10-05
Authors
Ayati, M
Tousi, V Najjaran
Sabokba, T
Vaziri-Nasab, H
Mehrkhani, F
Jamshidian, H
Dastan, J
Mosavi-Jarrahi, A R
Ghaffari, S R
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Abstract
Urinary cells obtained from voided urine specimens of 46 patients with urothelial carcinomas (UCs) and 10 normal individuals were analyzed with 3 different centromeric fluorescence in situ hybridization (FISH) probes. The overall sensitivity of cytology was 48.9% compared to 95.7%with the FISH technique. The minimum values were found for stage Ta and grade 1 (90.5 and 89.4) and sensitivity of FISH in other stages and grades was 100%. Chromosome 3 demonstrated the most frequent chromosomal abnormality in all samples (43%), followed by chromosome 17 (32%) and chromosome 7 (25%). There was a statistically significant association between the number of cell abnormalities in chromosome 17 and the tumour stage (p value=0.02). No relationship was found between the type of chromosomal abnormality and grade. Thus feasibility and reliability of a FISH based approach was confirmed for detection of UC in urine samples.
Description
Asian Pacific Journal of Cancer Prevention.
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Citation
Ayati M, Tousi VN, Sabokba T, Vaziri-Nasab H, Mehrkhani F, Jamshidian H, Dastan J, Mosavi-Jarrahi AR, Ghaffari SR. Molecular cytogenetic analysis of urothelial carcinomas using urine samples. Asian Pacific Journal of Cancer Prevention. 2008 Oct-Dec; 9(4): 687-90