Comparison of the RPHA and EIA techniques for the detection of HBs antigen among pregnant Thai women.
No Thumbnail Available
Date
2000-06-06
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Five hundred serum samples obtained from pregnant women attending an antenatal clinic in Bangkok were tested for HBsAg by reverse passive hemagglutination assay (RPHA) and enzyme immunoassay (EIA). It was found that 21 (4.2%) and 28 (5.6%) of the sera were positive by RPHA and EIA, respectively. The sensitivity and specificity of the RPHA were 75% and 100%, respectively, when using EIA as the standard method. The RPHA positive predictive value was 100% and the negative predictive value was 98.5%. Accuracy was 98.6%. This study showed that the RPHA was simple and required inexpensive equipment, making it suitable for mass screening. However, the possibility of false negative readings due to low levels of HBsAg should be kept in mind, especially in the blood transfusion practice.
Description
Published by the Allergy and Immunology Society of Thailand.
Keywords
Citation
Nathalang O, Arnutti P, Wansumrith N, Sriphaisal T. Comparison of the RPHA and EIA techniques for the detection of HBs antigen among pregnant Thai women. Asian Pacific Journal of Allergy and Immunology. 2000 Jun; 18(2): 115-7