Preparation of Gnathostoma protein by ultrafiltration method using Nanosep membrane.

Sugaroon, Suphan; Saksirisampant, Wilai; Kraivichian, Kanyarattana; Suwansaksri, Jamsai; Wiwanitkit, Viroj

Preparation of Gnathostoma protein by ultrafiltration method using Nanosep membrane.

Date

2003-09-25

Authors

Sugaroon, Suphan

Saksirisampant, Wilai

Kraivichian, Kanyarattana

Suwansaksri, Jamsai

Wiwanitkit, Viroj

Abstract

We report our experience with Gnathostoma protein preparation by the ultrafiltration method. Crude antigen was sonicated and ultrafiltrated using the Nanosep 100 K membrane. SDS-PAGE electrophoresis showed protein bands at 43, 41, 24, 22, 21, 19.5 kDa. Use of the ultrafiltration method can provide specific protein (24 kDa), similar to the non-ultrafiltration method, with the other 5 non-specific proteins. Using the non-ultrafiltration method, there were more (20) non-specific protein. The ultrafiltration method can be an alternative method for the preparation of protein, which can provide better results than non-ultrafiltration.

Description

The Southeast Asian Journal of Tropical Medicine and Public Health.

Citation

Sugaroon S, Saksirisampant W, Kraivichian K, Suwansaksri J, Wiwanitkit V. Preparation of Gnathostoma protein by ultrafiltration method using Nanosep membrane. The Southeast Asian Journal of Tropical Medicine and Public Health. 2003 ; 34 Suppl 2(): 121-2