Genetic differences of E gene of a Thai strain of Japanese encephalitis virus that determine small plaque size phenotype but not neurovirulence in suckling mice.
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Date
2008-05-21
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Abstract
Two small plaque variants of Japanese encephalitis virus (JEV), S4P9 and S9P10, were recovered from the wild type of JEV strain KE-093 using plaque purification in combination with the temperature-shift induction technique. Growth patterns of the S4P9 and S9P10 in BHK-21 cells as well as neurovirulence in suckling mice were similar to that of KE-093. An amino acid substitution, lysine for glutamic acid, was present in envelope protein at residue E-83 in the small plaque variants. This study shows that small plaque phenotype is not always associated with attenuation in vivo.
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The Southeast Asian Journal of Tropical Medicine and Public Health.
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Citation
Lorroengsil S, Chen A, Sällberg M, Pantuwatana S. Genetic differences of E gene of a Thai strain of Japanese encephalitis virus that determine small plaque size phenotype but not neurovirulence in suckling mice. The Southeast Asian Journal of Tropical Medicine and Public Health. 2008 May; 39(3): 387-93