Development of multiplex polymerase chain reaction for detection of Ehrlichia canis, Babesia spp and Hepatozoon canis in canine blood.
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Date
2009-01-28
Authors
Kledmanee, Kan
Suwanpakdee, Sarin
Krajangwong, Sakranmanee
Chatsiriwech, Jarin
Suksai, Parut
Suwannachat, Pongpun
Sariya, Ladawan
Buddhirongawatr, Ruangrat
Charoonrut, Phingphol
Chaichoun, Kridsada
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Abstract
A multiplex polymerase chain reaction (PCR) has been developed for simultaneous detection of canine blood parasites, Ehrlichia canis, Babesia spp and Hepatozoon canis, from blood samples in a single reaction. The multiplex PCR primers were specific to E. canis VirB9, Babesia spp 16S rRNA and H. canis 16S rRNA genes. Specificity of the amplicons was confirmed by DNA sequencing. The assay was evaluated using normal canine and infected blood samples, which were detected by microscopic examination. This multiplex PCR offers scope for simultaneous detection of three important canine blood parasites and should be valuable in monitoring parasite infections in dogs and ticks.
Description
The Southeast Asian Journal of Tropical Medicine and Public Health.
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Citation
Kledmanee K, Suwanpakdee S, Krajangwong S, Chatsiriwech J, Suksai P, Suwannachat P, Sariya L, Buddhirongawatr R, Charoonrut P, Chaichoun K. Development of multiplex polymerase chain reaction for detection of Ehrlichia canis, Babesia spp and Hepatozoon canis in canine blood. The Southeast Asian Journal of Tropical Medicine and Public Health. 2009 Jan; 40(1): 35-9