Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.
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Date
1989-12-01
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Abstract
A simple procedure was developed for spotting blood samples directly onto nylon membrane filter, without the necessity to treat samples with pronase or proteinase K, followed by hybridizing with 32P-labelled DNA probe, pUNK1-45. This probe detected specifically P. falciparum DNA and did not cross react with DNA from man, P. knowlesi, P. chabaudi or P. cynomolgi. The probe was sensitive to detect a parasitemia of 0.001% in 20 microliters of blood.
Description
The Southeast Asian Journal of Tropical Medicine and Public Health.
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Boonsaeng V, Chansiri K, Vilasineekul P, Wilairat P, Panyim S. Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application. The Southeast Asian Journal of Tropical Medicine and Public Health. 1989 Dec; 20(4): 519-22