Comparison of two IgG4 assay formats (ELISA and rapid dipstick test) for detection of brugian filariasis.
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Date
2003-12-30
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Abstract
Brugia malayi infection is endemic in several Asian countries. Filaria-specific IgG4 antibody detection based on BmR1 recombinant antigen has been shown to be sensitive and specific for the diagnosis of brugian filariasis. Two formats of the test has been reported ie indirect ELISA (BE) and rapid dipstick test (BR). Since different test formats use different amounts of sample and reagents which may affect its sensitivity and specificity, this study was performed to compare these two test formats in the detection of B. malayi. A total of 264 blinded serum samples from India and Malaysia were employed. Group 1 comprised 164 samples from actively infected individuals and group 2 comprised 100 samples from filaria non-endemic areas. Sensitivity was 96.3% (158/164) and 90.8% (149/164) for rapid test and ELISA respectively; chi-square p=0.00. Both test formats demonstrated 100% specificity. Therefore the rapid test format was equally specific but more sensitive than the ELISA format. The ELISA format would be able to demonstrate decline in IgG4 titer post-treatment while the rapid test would be very useful for screening and diagnosis in the field.
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The Southeast Asian Journal of Tropical Medicine and Public Health.
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Noordin R, Shenoy RK, Rahman RA. Comparison of two IgG4 assay formats (ELISA and rapid dipstick test) for detection of brugian filariasis. The Southeast Asian Journal of Tropical Medicine and Public Health. 2003 Dec; 34(4): 768-70