Preparation of monoclonal antibody against Angiostrongylus cantonensis antigen.
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Date
1989-03-01
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Abstract
Monoclonal antibodies (MAb) against A cantonensis were produced through fusion of immunised spleen cells from BALB/c mice with NS-1 myeloma cells at a ratio of 10:1. The successful fusion rate on the 3rd day of fusion was 90.1%. Ten MAb were characterised, six of which were IgG1 and the remaining four were IgG2a, IgG2b, IgM and IgA respectively. Among 6 IgG1 MAb, four were A. cantonensis-specific, of which three reacted to adult worm antigen only and one reacted to both adult worm and juvenile worm antigens. Two other IgG1 MAb showed cross-reaction with other helminthic antigens of Toxocara canis. Ascaris suum. Paragonimus westermani, Dirofilaria immitis, Anisakis Spp, Gnatostoma Spinigerum and Clonorchis sinensis.
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The Southeast Asian Journal of Tropical Medicine and Public Health.
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Yen CM, Chen ER, Kojima S, Kobayashi M. Preparation of monoclonal antibody against Angiostrongylus cantonensis antigen. The Southeast Asian Journal of Tropical Medicine and Public Health. 1989 Mar; 20(1): 119-24