In vivo culture of Cryptosporidium oocysts for laboratory use.
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Date
2006-05-06
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Abstract
In the present study, we describe in vivo cultivation to produce oocysts. Seven-day-old mice were orally infected with 100,000-120,000 Cryptosporidium oocysts. On day 8 post-infection, the mice were killed by ether, and the small and large intestines collected. A simple extraction procedure was used and purified using Ficoll gradient centrifugation. After purification, the oocysts were preserved in phosphate buffered saline with antibiotic at 4 degrees C before use.
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The Southeast Asian Journal of Tropical Medicine and Public Health.
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Sangloung C, Buddhirongawatr R, Tantawiwattananon N, Sukthana Y. In vivo culture of Cryptosporidium oocysts for laboratory use. The Southeast Asian Journal of Tropical Medicine and Public Health. 2006 ; 37 Suppl 3(): 18-20