ELISA for immunodiagnosis of human gnathostomiasis.
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Date
1985-06-01
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Abstract
An ELISA for immunodiagnosis of human gnathostomiasis using a crude water extract of third-stage larvae of G. spinigerum as antigen, and alkaline phosphatase labelled goat antihuman IgG in the indicator system was developed and evaluated. At the titre of 1:400 and above positive results were observed in 100% of 4 parasitological confirmed and 10 eosinophilic meningo-encephalitis (EME) typical of gnathostomiasis cases, 56% of 160 cutaneous migratory swelling cases, 33% of 24 cases with EME typical of A. cantonensis infections, 23% of 92 cases with other parasitic infections and 1.5% of blood donors. The overall sensitivity was 59% and specificity 84%. The predictive value was 77%. The results indicated that ELISA is potentially useful for immunodiagnosis of gnathostomiasis but improvement of sensitivity and specificity is needed.
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The Southeast Asian Journal of Tropical Medicine and Public Health.
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Citation
Suntharasamai P, Desakorn V, Migasena S, Bunnag D, Harinasuta T. ELISA for immunodiagnosis of human gnathostomiasis. The Southeast Asian Journal of Tropical Medicine and Public Health. 1985 Jun; 16(2): 274-9