Expression and purification of 30 kilodalton protein antigen of Ara- Burkholderia pseudomallei.
Loading...
Date
2009-03-28
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The 30 kDa protein of B. pseudomallei is found in virulent Ara- but not avirulent Ara+ strain. The gene was cloned in Escherichia coli JM105 employing pInIII-C2 vector. The open reading frame was 870 nucleotides with a guanine plus cytosine content of 69.9%. Arginine was the most abundant amino acid in the protein, having a PI of 12.65. Nucleotide sequence of the gene was 96% identical to B. pseudomallei 1710b chromosome II sequence CP000125.1, encoding an oxidoreductase of the short chain dehydrogenase/reductase family. The 30 kDa antigen was expressed as a maltose-fusion protein with a yield of 5.25 mg/l of bacterial culture.
Description
The Southeast Asian Journal of Tropical Medicine and Public Health.
Keywords
Citation
Rungpanich U, Pongsunk S, Ezaki T, Ekpo P. Expression and purification of 30 kilodalton protein antigen of Ara- Burkholderia pseudomallei. The Southeast Asian Journal of Tropical Medicine and Public Health. 2009 Mar; 40(2): 295-301