Detection of glutamate dehydrogenase enzyme activity in Plasmodium falciparum infection.

Rodriguez-Acosta, A; de Dominguez, N; Aguilar, I; Girón, M E

Detection of glutamate dehydrogenase enzyme activity in Plasmodium falciparum infection.

Date

1999-04-14

Authors

Abstract

We describe the separation of an active glutamate dehydrogenase [GDH (NADP+)] enzyme from the plasma of patients with P. falciparum infection using columns of sepharose anti-GDH (NADP+) of Proteus spp. The activity of this enzyme was also detected in P. falciparum culture supernatant. The parasitic origin of this enzyme was suggested by western blot analysis using anti-P. falciparum culture supernatant and anti-whole parasite antibodies. The differential inhibition of the P. falciparum GDH (NADP+) indicates that some epitopes recognised by the antibodies in both preparations may be different. The determination of P. falciparum GDH (NADP+) activity could be developed into a specific technique for the diagnosis of falciparum malaria.

Citation

Rodriguez-Acosta A, de Dominguez N, Aguilar I, Girón ME. Detection of glutamate dehydrogenase enzyme activity in Plasmodium falciparum infection. Indian Journal of Medical Research. 1999 Apr; 109(): 152-6