D-amino acid oxidase: its potential in the production of 7-aminocephalosporanic acid.
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1999-02-25
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Abstract
D-Amino acid oxidase (DAAO) used in the preparation of alpha-keto acids, in the determination of D-amino acids and in the resolution of racemic mixture of amino acids is produced by a wide range of microorganisms. In the recent past this enzyme is being recognized for its potential in the commercial production of 7-aminocephalosporanic acid (7-ACA), a starting material for various semisynthetic cephalosporins. Though this enzyme is widespread among microorganisms, very few microbial species have been explored for the production of 7-ACA; this is because cephalosporin C is quantitatively deaminated by limited microbial DAAOs. Comparison of physico-chemical properties of enzyme preparations indicate wide variations, however in general DAAOs are specific for D-configuration of amino acids. Both immobilized enzyme and cell preparations are developed for its various applications. The advantages of DAAO in the production of 7-ACA are discussed.
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Mujawar SK. D-amino acid oxidase: its potential in the production of 7-aminocephalosporanic acid. Hindustan Antibiotics Bulletin. 1999 Feb-Nov; 41(1-4): 1-14