Efficacy of voriconazole and amphotericin B in corneal preservative media
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Date
2022-01
Journal Title
Journal ISSN
Volume Title
Publisher
All India Ophthalmological Society
Abstract
Purpose: To evaluate the efficacy of voriconazole and amphotericin B in McCarey–Kaufman (MK) media. Methods: MK media vials were supplemented with either voriconazole at 1, 2, 20, 50, 100 ?g/mL or amphotericin B at 0.5, 1, 2, 10, 20 ?g/mL. The standard inoculum of the American Type Culture Collection (ATCC) strain of Candida albicans, Aspergillus flavus, and Fusarium keratinoplasticum was added to the set of vials. The efficacy outcomes were calculated as ‘viable fungal colony counts’ determined from the samples taken on Days 0 and 4. MK media containing fungal inoculum but without antifungal supplements were used as control. Results: In the voriconazole arm, on Day 4, a reduction in the colony count was observed for Candida albicans (1 ?g/mL, 36%; 100 ?g/mL, 100%), Aspergillus flavus (1 ?g/mL, 53.8%; 100 ?g/mL, 80.4%), and Fusarium keratinoplasticum (1 ?g/mL, 39.0%; 100 ?g/mL, 72.2%). Similarly, in the amphotericin B arm, on Day 4, a reduction in the colony count was observed for Candida albicans (0.5 ?g/mL; 99.9%; 20 ?g/mL, 100%), Aspergillus flavus (0.5 ?g/mL, 65.2%; 20 ?g/mL, 84.8%), and Fusarium keratinoplasticum (0.5 ?g/mL, 90.1%; 20 ?g/mL, 100%). Conclusion: Compared to voriconazole, the addition of amphotericin B significantly reduces fungal contamination in MK media.
Description
Keywords
Antifungal, corneal preservative medium, MK medium
Citation
Das Sujata, Mitra Sanchita, Garg Prashant, Mallick Aparajita, Priyadarshini Smruti R, Sharma Savitr. Efficacy of voriconazole and amphotericin B in corneal preservative media. Indian Journal of Ophthalmology. 2022 Jan; 70(1): 90-94