Use of eluates of filter paper blood spots in ELISA for the serodiagnosis of leprosy.
No Thumbnail Available
Date
1989-05-01
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Blood samples were collected from 59 leprosy patients and 35 normal healthy subjects by veni-puncture and finger prick methods to obtain serum samples and blood spots on filter paper respectively. The serum samples at 1:300 dilutions and the eluates of dried blood spots at 1:40, 1:80, 1:160 and 1:320 dilutions were applied in ELISA to measure the antibody levels (IgM) against synthetic ND-O-BSA antigen. The antibody levels were found to be high in the multibacillary leprosy patients than the pauci-bacillary patients irrespective of whether serum samples or eluates were used. The OD values obtained at 1:160 dilution of the eluates were equivalent to that of values obtained at 1:300 dilution of the serum samples. The positivities differ in different dilution of the eluates, showing the highest in the 1:40 dilution and the lowest in the 1:320 dilution.
Description
Keywords
Citation
Dhandayuthapani S, Anandan D, Vasanthi B, Bhatia VN. Use of eluates of filter paper blood spots in ELISA for the serodiagnosis of leprosy. Indian Journal of Medical Research. 1989 May; 89(): 150-7