Residue Analysis of Oxytetracycline in Milk Sample by Two Different Chromatographic Methods and Determination of Ionization Constant Values
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Date
2020-08
Journal Title
Journal ISSN
Volume Title
Publisher
SCIENCEDOMAIN international
Abstract
In thepresent study, two analytical methods for the residue analysis of oxytetracycline in milk sample have been generated. InHPLC method, the analysis was performed on an X Terra RP-18 column at 25 °C with the mobile phase as methanol: water (20 : 80 )(v/v) modified to pH 5. For the second method capillary electrophoresis system was used. The analysis of oxytetracycline in milk sample could be achieved without using organic modifier in a 58 cm length capillary at a working voltage of 12 kV with 20 mM NaH2PO4-H3PO4(pH 7) by capillary electrophoresis. Tetracycline was used as internal standard in both methods. The results calculatedfrom both methods were compared to each other. The calculated data for drugs was checked with the data predicted by the SPARC on-line pKa estimator
Description
Keywords
Oxytetracycline, milk, residue analysis, HPLC, capillary electrophoresis
Citation
Sardoğan Seyfi, Şanlı Senem, Sardoğan Barış, Atalay Barış. Residue Analysis of Oxytetracycline in Milk Sample by Two Different Chromatographic Methods and Determination of Ionization Constant Values. Journal of Pharmaceutical Research International. 2020 Aug; 32(15): 1-9