Antimicrobial resistance pattern of microorganisms isolated and identified from Godavari River across the mass gathering event
Loading...
Date
2019-10
Journal Title
Journal ISSN
Volume Title
Publisher
Indian Academy of Sciences
Abstract
The Kumbh Mela is considered the world’s largest mass gathering event (MGE). It represents a unique anthropogenicburden on the river ecosystem and may confer antimicrobial resistance among microbial communities. The present studyexplains the characterization of microbial isolates associated with Godavari River under the advent of the mass bathingevent using culture dependent approach. The isolates were identified by sequencing of 16S rRNA gene and ITS region.Further, the isolates were screened for antibiotic susceptibility against 22 antibiotics using disc diffusion method. A total of63 bacterial and 21 fungal isolates were isolated under the temporal variation of the event. Kocuria and Staphylococcuswere the most prevalent bacterial genera in the samples collected before and during the event, while Meyerozyma andCandida predominate among fungal communities. A group of antibiotics impeding the bacterial protein synthesis werefound to be most effective against C67% of bacterial isolates. However, C37–67% of isolates could escape the action ofinhibitors of bacterial cell wall synthesis. Moreover, bacterial isolates belonging to genera Acinetobacter, Corynebacteriumand Brevibacterium showed higher resistance towards the antibiotics. Observations from the current study suggest likelymicrobial taxa as targets to mitigate the waterborne infections during the MGE.
Description
Keywords
Antibiotic susceptibility testing, Godavari River, mass gathering event, molecular identification
Citation
Jani Kunal, Bandal Jayashree, Rale Vinay, Shouche Yogesh, Sharma Avinash. Antimicrobial resistance pattern of microorganisms isolated and identified from Godavari River across the mass gathering event. Journal of Biosciences. 2019 Oct; 44(5): 1-6