RP-HPLC analysis of methanol extract of Viscum articulatum
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Date
2020-07
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Background: Viscum articulatum Burm. (Family: Loranthaceae) is commonly known as mistletoe. Inayurveda, the plant parts are used in “Kapha”, “Vata”, diseases of the blood, ulcer, and epilepsy. The plantparts are also used in urinary tract infection and wound infection. The plant contains five triterpenoidssuch as a-amyrin, lupeol, betulin, betulinic acid and oleanolic acid, exhibiting several pharmacologicalactivities including antimicrobial, anti-HIV, antitumor, antiviral activity.Objective: To ensure the content of uniformity of oleanolic acid, a RP-HPLC method has been developedfor estimation of oleanolic acid in V. articulatum aerial part.Material and methods: The RP-HPLC method was carried out in reverse phase C18 column, using methanol and water as mobile phase in the ratio of 95:5 (v/v), at the flow rate of 1 mL/min. The pH of aqueousphase was adjusted 3.2 with 1% (v/v) glacial acetic acid. The lmax was set at 210 nm.Results: The retention time of oleanolic acid was found at 21.5 ± 0.05 min. The linearity of the response wasfound to be 10e800 mg/mL. The coefficient of determinants of oleanolic acid was found to be (r2) 0.995 andequation Y ¼ 19462X þ 16,172. The LOD and LOQ were found to be for oleanolic acid (1.96% w/w) 0.197 ± 0.63and 0.623± 0.87 mg/mL, respectively. The developed method was accurate, specific, precise and reproducible.Conclusion: This RP-HPLC may be useful for quantitative estimation of the chemical constituents presentin the plant extract as well as the quality assessment of the herbal product.
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Keywords
RP-HPLC, Viscum articulatum Burm, Oleanolic acid, Ayurveda, Method validation
Citation
Nag Mrinmoy, Amit Kar, Chanda Joydeb, Mukherjee Pulok K.. RP-HPLC analysis of methanol extract of Viscum articulatum. Journal of Ayurveda and Integrative Medicine. 44013; 11(3): 277-280