Influence of Media Supplementation with Alpha Tocopherol and/or Epigallocatechin Gallate on in vitro Maturation and Subsequent Fertilization of Bovine Oocytes

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Date
2019-12
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Publisher
Association of Mastitis
Abstract
The present study was planned to determine the effect of Epigallocatechin Gallate (EGCG), Alpha tocopherol and their combination as an antioxidant in TCM-199 media for in vitro maturation (IVM) and in vitro fertilization (IVF) in bovine oocytes. Cumulus-oocyte complexes (COCs) were aspirated from the ovaries derived from slaughter house and in vitro cultured in three groups using TCM-199 supplemented with EGCG @10 μM, Alpha tocopherol @100 μM, and Combination (EGCG @10 μM plus Alpha tocopherol @100 μM). Oocytes of a control group were matured in TCM-199 medium without any treatment. After IVM, cumulus-free oocytes were co-incubated with frozen-thawed spermatozoa for 15–18 h. Compared to no addition, the presence of EGCG @10 μM in medium during IVM significantly (p<0.05) increased the proportion of maturation and fertilization rate. Combination produced significantly higher percentage of in vitro matured bovine oocytes compared to the alpha tocopherol @100 μM alone. The results suggest that EGCG @10 μM in IVM medium had better effect than Alpha tocopherol alone and Combination on in vitro maturation and subsequent fertilization of bovine oocytes.
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Keywords
Alpha tocopherol, Epigallocatechin gallate, IVM, IVF, TCM-199
Citation
Singh W.L., Barua P.M., Sonowal J.. Influence of Media Supplementation with Alpha Tocopherol and/or Epigallocatechin Gallate on in vitro Maturation and Subsequent Fertilization of Bovine Oocytes. Journal of Animal Research. 2019 Dec; 9(6): 863-868