Toxigenic Profiling of Enterotoxin-Producing Bacillus cereus Isolated from Marketed Raw Chicken Meat and Human Subjects by Triplex and Multiplex PCR
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Date
2019-12
Journal Title
Journal ISSN
Volume Title
Publisher
Association of Mastitis
Abstract
Bacillus cereus incorporates the most important group of endospore-forming micro organism and can cause emetic and diarrheal food poisoning. A total of 42 B. cereus strains isolated from marketed raw chicken meat and human subjects swab samples were assessed by a triplex and multiplex PCR for the presence of enterotoxin genes. The detection rate of nheB, hblA, hblD, cytK, nheA,CER, hblC and entFM enterotoxin genes among all B. cereus strains was 83.33%, 80.95%, 69.04%, 21.42%, 47.61%, 0%, 61.90%, and 92.85% respectively. Enterotoxigenic profiles were determined in enterotoxin-producing strains showed 19 different patterns. The results offer essential information on toxin genes prevalence and toxigenic profiles of B. cereus from sources of origin. The present study was taken into consideration about extreme fitness danger for public health and insuring extra ability in difficulty to food safety amongst all B. cereus group members. Also, there may be need for extensive and continuous tracking of food products embracing both emetic toxin and enterotoxin genes.
Description
Keywords
Bacillus cereus, enterotoxigenic profiling, PCR, enterotoxin, marketed, Chicken, Meat
Citation
Suthar A.P., Kumar R., Savalia C.V., Patel N.M., Kalyani I.H.. Toxigenic Profiling of Enterotoxin-Producing Bacillus cereus Isolated from Marketed Raw Chicken Meat and Human Subjects by Triplex and Multiplex PCR. Journal of Animal Research. 2019 Dec; 9(6): 821-829