Brucyclin, a novel antimicrobial peptide structurally designed from the antibacterial plant peptide, Brucin
Loading...
Date
2020-02
Journal Title
Journal ISSN
Volume Title
Publisher
Open Science Publishers LLP
Abstract
A novel antimicrobial cyclic peptide, Brucyclin, was rationally designed from the original antibacterial plant peptide,Brucin. The chemically synthesized Brucyclin consists of amino acid sequence; (NH2- Gln-His-Thr-Leu-Cys-MetCys-Gly-Gly-Ala-Thr-Trp-COOH), with a molecular mass of m/z 1290. In the antimicrobial assay with 31 strainsof pathogenic microorganisms, the peptide exhibited the most antimicrobial activity with a minimum inhibitoryconcentration (MIC) values ranging from 50 to 100 μg/ml against two strains of Gram-negative bacteria (Vibriocholera non O1, non O139 and Klebsiella oxytoca), one strain of Gram-positive bacterium (Bacillus subtilis), andone strain of yeast (Candida albicans), respectively. Structural analysis of Brucyclin indicated that it has a neutralcharge with a hydrophobicity ratio of 50% and pI value of 6.72, respectively. The results from this study suggested thatthe Brucyclin is a new antibiotic peptide that might be an alternative potent drug for treatment of various infectiousdiseases caused by pathogenic microorganisms.
Description
Keywords
Antibacterial activity, antimicrobial peptide, Brucin, Brucyclin, cyclic peptide.
Citation
Thakorn Sornwatana, Nuanchawee Wetprasit, Somchoke Traewachiwiphak, Thanawat Phongsak, Sittiruk Roytrakul. Brucyclin, a novel antimicrobial peptide structurally designed from the antibacterial plant peptide, Brucin. Journal of Applied Pharmaceutical Science. 2020 Feb; 2020 Feb: 012-020