Antiproliferative and apoptosis effect of hyptolide from Hyptis pectinata (L.) Poit on human breast cancer cells
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Date
2020-02
Journal Title
Journal ISSN
Volume Title
Publisher
Open Science Publishers LLP
Abstract
Hyptolide was isolated from the leaves of Hyptis pectinata (L.) Poit and was studied in order to discover and develop ananticancer drug. Hyptolide was obtained as a crystal of 87°C–88°C melting point. Spectroscopic identification resultsshow a wave number at 1,735 cm−1 indicating the presence of α,β-unsaturated δ-lactone. Gas chromatography-massspectrometry (GC-MS) analysis provides a single peak in the retention time of 11.701 by m/z value at 239, whichindicates explicitly hyptolide. The objective of this research was to evaluate the hyptolide’s mechanism of cytotoxicon MCF-7 human breast cancer cells in positive estrogen receptor. The assay test to 3-(4,5-dimethylthiazol-2-yl)-2,5-dphenyl tetrazolium bromide (MTT) showed that hyptolide exhibited cytotoxic effects on MCF-7 and T47D breastcancer cells with an IC50 value of 76.76 and 181.55 µg/ml, respectively. Interestingly, the treatment of hyptolide for 24,48, and 72 hours decreased cell viability on MCF-7 with dose- and time-dependent manner compared to untreated cells.Results of acridine orange-ethidium bromide multiple staining assay revealed that hyptolide induced apoptosis in a dosedependent manner. It can be concluded that hyptolide possesses antiproliferative effects through apoptosis induction
Description
Keywords
Hyptis pectinata (L.) Poit, hyptolide, cytotoxic, apoptosis, antiproliferative, MCF-7 cells.
Citation
Suzery Meiny, Cahyono Bambang, Amalina Nur Dina. Antiproliferative and apoptosis effect of hyptolide from Hyptis pectinata (L.) Poit on human breast cancer cells. Journal of Applied Pharmaceutical Science. 2020 Feb; 2020 Feb: 001-006