Antiproliferative activity of longan (Dimocarpus longan Lour.) leaf extracts
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Date
2019-05
Journal Title
Journal ISSN
Volume Title
Publisher
Open Science Publishers LLP
Abstract
Longan (Dimocarpus longan Lour.) belongs to Sapindaceae family. We examined the antiproliferative activity oflongan leaf extracts against cancer-derived cell cell lines in vitro. The tested samples were water extract, ethanolextract, n-hexane fraction, ethyl acetate fraction, and water fraction of longan leaf. Cytotoxicity test is against brineshrimps that was screened using Brine Shrimp Lethality Test. Antiproliferative activity assay on WEHI-164 cells(mouse fibrosarcoma cancer cell), THP-1 cells (human peripheral blood acute monocyte cell), and vero cells (noncancer or normal cell) that was conducted using hemocytometer with Trypan Blue Dye exclusion. The 50% lethalityconcentration (LC50) value of water extract, ethanol extract, n-hexane fraction, ethyl acetate fraction, and waterfraction were 854.64, 305.81, 446.55, 1313.44, and 1621.8 µg/ml. Ethanol extract exhibited significant cytotoxicdue to the lowest LC50 value. Ethanol extract was then used for further examination. The highest antiproliferativeactivity was achieved 44.93% by 600 µg/ml ethanol extract on WEHI-164 and 57.45% by 500 µg/ml ethanol extracton THP-1. It was significantly equal to doxorubicin antiproliferative activity. Ethanol extract dose had low effect tovero cells. This present study confirmed that the longan leaf ethanol extract possess marked antiproliferative activityon cancer-derived cell lines.
Description
Keywords
Antiproliferative, cancer cell lines, cytotoxicity, in vitro, longan leaf.
Citation
Puspita Ratna, Bintang Maria, Priosoeryanto Bambang Pontjo. Antiproliferative activity of longan (Dimocarpus longan Lour.) leaf extracts. Journal of Applied Pharmaceutical Science. 2019 May; 2019 May: 102-106