Antimicrobial activity screening of endophytic fungi extracts isolated from brown algae Padina sp.
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Date
2019-04
Journal Title
Journal ISSN
Volume Title
Publisher
Open Science Publishers LLP
Abstract
This study aims to evaluate the antimicrobial activities of endophytic fungi from marine brown algae Padina sp.collected from Nirwana Beach, Padang, West Sumatera, Indonesia. The isolation of endophytic fungi was conductedusing dilution method with Sabouraud Dextrose Agar (SDA) + Chloramphenicol as a growth medium. Nine fungistrains have been isolated from this alga. Purely isolated fungi were cultivated using rice as a medium at roomtemperature for 3–4 weeks. The secondary metabolite produced by fungi was extracted using ethyl acetate (EtOAc)as a solvent. The antimicrobial activity of EtOAc extracts was tested against Staphylococcus aureus, Escherichiacoli, and Candida albicans by using the agar diffusion method. In this research, nine endophytic fungi were isolatedfrom the brown marine algae Padina sp. The results of antimicrobial activity screening showed that one fungal isolate(Nita3) was selected as the most active against S. aureus, E. coli, and C. albicans with a diameter of inhibition zone of20.98 ± 1.56 mm, 17.98 ± 6.58 mm, and 13.60 ± 0 mm, respectively. This selected fungus was identified molecularlyas Trichoderma harzianum. We conclude that T. harzianum can be a source of antimicrobial compounds. However,continuous research is needed to prove its bioactive action
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Keywords
Antimicrobial activity, brown algae, marine algaederived fungi, molecular characterization, Trichoderma harzianum, Padina sp
Citation
Handayani Dian, Ananda Nita, Muh. Ade Artasasta, Ruslan Rustini, Fadriyanti Okmes, Trina Ekawati Tallei. Antimicrobial activity screening of endophytic fungi extracts isolated from brown algae Padina sp.. Journal of Applied Pharmaceutical Science. 2019 Apr; 2019 Apr: 009-013