Elicitation effect on the production of asiaticoside and asiatic acid in shoot, callus, and cell suspension culture of Centella asiatica
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Date
2019-06
Journal Title
Journal ISSN
Volume Title
Publisher
Open Science Publishers LLP
Abstract
Centella asiatica is an important medicinal plant which contains various phytocompounds. Asiatic acid and asiaticosideare two major compounds which are responsible for its various pharmaceutical activities. The present study analyzesthe effect of elicitor, i.e., methyl jasmonate on the synthesis of asiaticoside and asiatic acid (ATA) in shoot, callus, andcell suspension cultures of C. asiatica. A high-performance liquid chromatography analysis showed that the elicitationwith 100 µM concentration of methyl jasmonate enhanced asiaticoside content by 69-fold in callus culture, 39-fold inshoot cultures, and ATA by 1.9-fold in cell suspension culture. Thus, elicitation with methyl jasmonate is an effectivemethod of increasing the rate of biosynthesis of asiaticoside and ATA in plant cell cultures of C. asiatica
Description
Keywords
Centella asiatica, asiaticoside, asiatic acid, cell suspension culture, methyl jasmonate, shoot cultures.
Citation
Krishnan M. Laxmi, Roy Arpita, Bharadvaja Navneeta. Elicitation effect on the production of asiaticoside and asiatic acid in shoot, callus, and cell suspension culture of Centella asiatica. Journal of Applied Pharmaceutical Science. 2019 Jun; 2019 Jun: 067-074