Sequestration and purification of essential enzyme from Pleurotus florida compost waste and its application studies
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Date
2020-01
Journal Title
Journal ISSN
Volume Title
Publisher
Open Science Publishers LLP
Abstract
Spent oyster mushroom (Pleurotus florida) compost as tremendous source for isolation of industrial significantenzymes such as amylase, protease, and cellulase. This study was conducted to achieve efficient extraction oflignocellulolytic enzymes amylase (EC 3.2.1.1), cellulase (EC 3.2.1.4), and protease (EC 3.4.21.14) from spent oystermushroom (P. florida) compost waste. Optimal enzyme recovery was achieved when spent oyster mushroom compostwastes and concentrated by acetone precipitation. The purification was performed by column chromatography. Theenzymes such as cellulase, amylase, and protease released from oyster mushroom (P. florida) compost waste wereshowed activities of 15.78 U/ml, 3.42 U/ml, and 0.042 U/ml, respectively. These were utilized in various industrialand environmental applications such as starch processing in potato waste from food industry using amylase andbiotreatment of cotton waste using cellulase.
Description
Keywords
Spent mushroom compost, Amylase, Cellulase, Protease, Starch processing, Biotreatment
Citation
Devi S Karthika, N Prabhu, S Monika, NM Swapna, S Vaishali, T Gajendran. Sequestration and purification of essential enzyme from Pleurotus florida compost waste and its application studies. Journal of Applied Biology & Biotechnology. 2020 Jan; 2020 Jan: 64-68