High-density culture of Vero cells in novel perfusion bioreactor system
Loading...
Date
2019-09
Journal Title
Journal ISSN
Volume Title
Publisher
Open Science Publishers LLP
Abstract
Viruses are the obligatory intracellular parasites infecting microbes, plants, animals, and humans. They aredead outside host cell but can take-over the host’s cell machinery as soon as they are into it. Several studies oninhibitor compounds have been done for animal viruses including those that are affecting humans, but thereis inadequacy in terms of research and literature for plant viruses that are responsible for losses in crop yieldand quality loss all across the globe. This could be focal point to study plant viruses, their transmission andpathogenicity, and to establish widely used, effective, and advanced approaches for their control. The purposeof this review is to discuss various approaches to control plant viruses that have been developed and applied tocombat plant viral infections. We have divided these approaches into two categories conventional (meristemtip culture, cryotherapy, thermotherapy, and chemotherapy) and advanced (nucleic acid-based approacheslike RNA Silencing, cross-protection, transgenic plants, gene pyramiding, and protein-protein interaction).Moreover, we have discussed and compared the principles, methodologies, advantages, and disadvantages ofeach technique. The approaches have been explored to promote their application in best suited way on variousplants to control viral diseases and to improve food crops quality with increase in production.
Description
Keywords
SUBs, Vero cell culture, perfusion bioprocessing, 3D cell carriers, non-woven polyester microfibers, fixed-bed and packed-bed bioreactors, PLC
Citation
Patel Ravindra, Dasgupta Debjani, Vernekar Madhavi. High-density culture of Vero cells in novel perfusion bioreactor system. Journal of Applied Biology & Biotechnology. 2019 Sep; 2019 Sep: 50-55