Investigating The Role Of Nf-Κb, Cox-1, Cox-2, Comt, Il-10, Il-6 And Tnf-Α In Modulating Anti-Nociceptive Activity Of Methanolic Extract Of Entada Phaseoloides
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Date
2020-08
Journal Title
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Publisher
Innovare Academic Sciences Pvt. Ltd.
Abstract
Objective: To investigate the analgesic activity of methanolic extract of Entada phaseoloides (MEEP) along with its molecular mechanistic pathway.
Methods: Swiss albino female mice and Wistar rats of either sex were administered orally with MEEP extracts (100, 200 and 400 mg/kg) and pentazocine, tramadol and diclofenac sodium, as standard drugs. Following administration, anti-nociceptive activity was evaluated using an acetic acid-induced writhing test, Eddy’s hot plate, and hot water immersion test. Serum was collected for molecular expression of various proteins and genes using Reverse Transcriptase PCR and Western Blotting.
Results: Acetic acid writhing test, a frequently used method to assess peripheral analgesic activity, revealed that MEEP reduced peripherally induced pain in a dose-dependent manner. Likewise, Eddy’s hot plate and hot water immersion methods, often implicated for testing central analgesic activities, showed that MEEP is bestowed with the capability to counteract analgesia in a dose and time-dependent manner. Pro-inflammatory cytokines and factors like COX-2, IL-6, TNF-α, and NF-κB that cause inflammatory responses and pain were significantly reduced, suggesting its analgesic and anti-inflammatory potential. This analgesic and the anti-inflammatory role played by MEEP is also supported by the up-regulation of anti-inflammatory cytokine IL-10 and COMT and COX-1 enzyme demonstrated no significant difference between the groups.
Conclusion: The study revealed the weak peripheral and potent central analgesic property MEEP by modulating pro-inflammatory and anti-inflammatory pathways.
Description
Keywords
Analgesic, Cytokines, Diclofenac sodium, Entada phaseoloides, Pentazocine, Tramadol
Citation
Barua C. C., Barbhuiya S. M. A. A., Buragohain L., Kakati A., Barua A. G.. Investigating The Role Of Nf-Κb, Cox-1, Cox-2, Comt, Il-10, Il-6 And Tnf-Α In Modulating Anti-Nociceptive Activity Of Methanolic Extract Of Entada Phaseoloides. International Journal of Pharmacy and Pharmaceutical Sciences. 2020 Aug; 12(8): 172-179