Anti-Tumour Activity Of Bromopyrrole Alkaloids Against Human Breast Tumour (MCF-7) Through Apoptosis Induction
Date
2019-06
Journal Title
Journal ISSN
Volume Title
Publisher
Innovare Academic Sciences Pvt. Ltd.
Abstract
Objective: The present study was conducted to screen the cytotoxicity and initiation of apoptosis in MCF-7 human breast tumour cells using the Indian sponge Acanthostylotela cornuta of the Gulf of Mannar.
Methods: The crude methanol extract of A. cornuta was fractionated using a reversed phase silica gel column chromatography. The purity of the active fraction of bromopyrrole alkaloids was confirmed with Gas Chromatography-Mass Spectrometry (GCMS). The bromopyrrole alkaloids induce apoptotic changes in MCF-7 cells were studied with electrophoresis, caspase assay, and different staining analysis. The MCF-7 cells were analyzed by Flow cytometry to determine their DNA content.
Results: Bromopyrrole alkaloids of A. cornuta showed cytotoxicity against MCF-7 human breast tumour cells with the IC50 value of 8.0μg/ml. The bromopyrrole compounds induced cells exhibited scatted red fluorescence, showing the presence of several residual bodies and condensation of chromatin. Caspase-3, pro-caspase-9, caspase-9 and Poly-ADP-Ribose-Polymerase (PARP) activity were occurred in bromopyrrole alkaloids treated tumour cell. The cell cycle arrest is conceivable that the compound prevented the progression of cell cycle through the G phase resulting from inhibition of survival, leading them to undergo apoptosis.
Conclusion: Bromopyrrole alkaloids of A. cornuta possess antitumor activity which was arrested the G phase in cell cycle that clearly indicated its nature as that of antitumour drugs.
Description
Keywords
Marine sponge, Acanthostylotela cornuta, Antitumour, Breast tumour, Apoptosis
Citation
Manikandan S., Ganesapandian S., Singh M., Kumaraguru A. K.. Anti-Tumour Activity Of Bromopyrrole Alkaloids Against Human Breast Tumour (MCF-7) Through Apoptosis Induction. International Journal of Pharmacy and Pharmaceutical Sciences. 2019 Jun; 11(6): 94-100