Use of TaqMan Array card for the detection of respiratory viral pathogens in children under 5 years old hospitalised with acute medical illness in Ballabgarh, Haryana, India
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Date
2019-03
Journal Title
Journal ISSN
Volume Title
Publisher
Indian Association of Medical Microbiologists
Abstract
Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1� human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reverse transcriptase polymerase chain reaction (rRT-PCR). They were retested using TaqMan Array Card (TAC), a micro-fluidic system, capable of simultaneous multi-pathogen testing, to evaluate its sensitivity and specificity against monoplex rRT-PCR. TAC showed high sensitivity (71%�0%) and specificity (98%�0%) for these viruses in comparison to monoplex rRT-PCR. Multi-specimen detection with high sensitivity and specificity makes TAC a potentially useful tool for both surveillance and outbreak investigations.
Description
Keywords
Micro-fluidic card, sensitivity, specificity, viral pathogen diagnosis
Citation
Gaur B, Saha S, Iuliano A D, Rai SK, Krishnan A, Jain S, Whitaker B, Winchell J, Lal RB, Broor S. Use of TaqMan Array card for the detection of respiratory viral pathogens in children under 5 years old hospitalised with acute medical illness in Ballabgarh, Haryana, India. Indian Journal of Medical Microbiology. 2019 Mar; 37(1): 105-108