Please use this identifier to cite or link to this item: http://imsear.searo.who.int/jspui/handle/123456789/183465
Title: Immunohistochemical Study of Matrix Metalloproteinase-9 in Medial Collateral Ligament Epiligament in Rat Knee after Grade III Injury.
Authors: Iliev, Alexandar
Georgiev, Georgi P
Kotov, Georgi
Dimitrova, Iva N
Lina, Malinova
Rashev, Pavel
Tsankov, Lubomir
Karabinov, Vesselin
Landzhov, Boycho
Department of Anatomy, Histology, and Embryology, Medical University of Sofia, Bulgaria
Department of Orthopaedics and Traumatology, University Hospital Queen Giovanna-ISUL, Medical University Sofia, Bulgaria
Department of Cardiology, University Hospital “St. Ekaterina”, Medical University of Sofia, Bulgaria
Department of Immunology of Reproduction, Institute of Biology and Immunology of Reproduction – “Acad. K. Bratanov”, Bulgarian Academy of Sciences, Sofia, Bulgaria
Department of Image Diagnostic, University Hospital "Dr. G. Stransky", Medical University of Pleven, Bulgaria
MHAT National Cardiology Hospital, Clinics of Neurology, Sofia, Bulgaria
Keywords: Matrix metalloproteinase-9
medial collateral ligament epiligament, grade III injury
immunohistochemical study
Issue Date: Jan-2017
Citation: Iliev Alexandar, Georgiev Georgi P, Kotov Georgi, Dimitrova Iva N, Lina Malinova, Rashev Pavel, Tsankov Lubomir, Karabinov Vesselin, Landzhov Boycho. Immunohistochemical Study of Matrix Metalloproteinase-9 in Medial Collateral Ligament Epiligament in Rat Knee after Grade III Injury. Academia Anatomica International. 2017 Jan-June; 3(1): 20-25.
Abstract: Background: In the present study, we conduct an immunohistochemical analysis to evaluate the expression of matrix metalloproteinase-9 (MMP-9) in the epiligament (EL) after grade III injury of the medial collateral ligament (MCL) in an experimental model of a rat knee. Methods: Twelve 8-month old male Wistar rats were used in this study. Three animals were used as controls, while the remaining nine underwent grade III injury of the MCL. The MMP-9 immunoreactivity was evaluated on the 8th, 16th and 30th day after injury. Results: We observed an intensive expression of the enzyme in all periods after injury in contrast with the control group. We also discovered that the main source of matrix metalloproteinase-9 was localized in the epiligament tissue. Immunoreactivity was highest and homogeneously distributed on the 8th day and gradually diminished, concentrating on the EL-ligament border and the perivascular zones on the 30th day. Conclusion: We present the first immunohistochemical study of the expression and distribution of the enzyme MMP-9 in the EL of the MCL and track the changes in enzyme activity on the 8th, 16th and 30th day after damage.
URI: http://imsear.searo.who.int/jspui/handle/123456789/183465
ISSN: 2455-5274
Appears in Collections:Academia Anatomica International

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