Leptin promoter variant G2548A is associated with serum leptin and HDL-C levels in a case control observational study in association with obesity in a Pakistani cohort.
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2016-06
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Abstract
Leptin is a protein hormone synthesized by adipocytes and is involved in the regulation of food intake and energy
expenditure. We hypothesized that any change in the promoter sequence can affect the expression of the gene and
hence leptin protein levels in the serum. The aim of the current study was to investigate the relationship of such a
promoter variant of the leptin gene, G-2548A polymorphism, with obesity and its effect on various anthropometric
and metabolic parameters in a Pakistani cohort consisting of 250 obese and 225 non-obese control subjects. Body
weight, height, waist circumference (WC), hip circumference (HC) and blood pressure (BP) were measured by
standard methods and levels of fasting blood glucose (FBG), total cholesterol, triglycerides, HDLC, LDLC, and leptin
were determined. Genotyping was done by polymerase chain reaction-restriction fragment length polymorphism
(PCR-RFLP). The results showed that the LEP G-2548A polymorphism showed significant association with obesity
in Pakistan. In addition, the polymorphism showed association with weight, height, BMI, WC, HDLC and serum
leptin levels. The findings suggest that the leptin promoter G-2548A variant may play its part in the progression to
obesity by not only affecting the body’s fat distribution but also by changing the serum leptin and HDLC levels.
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Leptin, obesity, Pakistan, promoter
Citation
Shabana, Hasnain Shahida. Leptin promoter variant G2548A is associated with serum leptin and HDL-C levels in a case control observational study in association with obesity in a Pakistani cohort. Journal of Biosciences. 2016 June; 41(2): 251-255.